The online retailer posted $847m (£585m) in revenue for the three months ended 31 March, a 21% increase over $700m in revenue reported for the same period in 2001, the company said in a statement.
Including restructuring charges and other items, Amazon reported a net loss for the quarter of $23m (£16m), or six cents per share. In the same quarter last year it posted a net loss including charges of $234m, or 66 cents per share.
Excluding charges, Amazon reported a net loss for the quarter just ended of $5m, or 1 cent per share, the company said.
Amazon posted its first-ever net profit in January when it released results for its fourth fiscal quarter of 2001.
The company said it plans to return to profitability this year, forecasting a 15% to 22% increase in revenue for the second quarter and pro forma operating income of between $5m (£3.5m) and $15m (£10.5m), according to the statement.