At the centre of the dispute is a technology used in Akamai's EdgeSuite product that sidesteps Internet congestion by finding alternative paths for information to travel.
Cable & Wireless claims its Internet optimal routing technology is protected by its US Patent 6,275,470, which covers the Host-to-Host Adaptive Routing Protocol (HHARP).
It asked a US District Court judge for a preliminary injunction to prevent Akamai from selling EdgeSuite until it removes the HHARP technology.
The request is part of a Cable & Wireless lawsuit filed in August that charged Akamai and its affiliate Sockeye Networks with infringing on HHARP.
However, Akamai spokesman Jeff Young said: "We believe that we do not infringe [on the technology patent]. The claims are totally without merit."
Young added that the injunction had nothing to do with preventing Akamai from selling EdgeSuite.